– For biological and medical applications fluorescence microscopes are used to observe certain parts of living cells and tissues with help of fluorophores.
-One or more fluophores are added to the parts of the specimen to be observed.
-When the fluorophores are exposed to so-called exicitation light, the excitation energy is absorbed by the fluorophore that start emitting so-called emission light that can be observed by the microscope.
-One can distinguish tumor cells from other cells, follow biological processes or prove the presence or absence of antibodies, etc
– The Oxion microscopes for fluorescence are equipped with an epi-illumination with a 100 W mercury vapor lamp.
-The fluorescence attachment have a rail for maximum 4 filter cubes.
-The FluoLed model is equipped with a 455 nm LED and one filter cube, especially developed for efficient and fast detection of tuberculosis by the Auramine-O fluorochrome.
-Other FluoLED wavelengths are also available.
HWF 10x/22 mm eyepieces. All eyepieces can be secured by an Allen screw
Binocular and trinocular Siedentopf type heads are with 30° inclined tubes. Interpupillary distance from 50 to 75 mm. Two ± 5 diopter adjustments. A unique rotating system allows the ergonomical positioning of both tubes in a high (431 mm) and in a low position (397 mm)
Revolving and reversed nosepiece for maximum 5 objectives. The fluorescence models with mercury vapor lamp are equipped with an orange UV protection shield
• Plan 4x/0.10, 10x/0.25, S40x/0.65 and S100x/1.25 oil immersion IOS objectives
• Plan fluarex 4x/0.10, 10x/0.25, S40x/0.65 and S100x/1.25 oil immersion IOS objectives
• Plan semi apochromatic fluarex 4x/0.10, 10x/0.25, S40x/0.65 and S100x/1.25 oil immersion IOS objectives
All S40x, S60x and S100x oil immersion objectives are spring loaded. All optics are anti-fungus treated and anti-reflection coated for maximum light throughput
Coaxial coarse and fine adjustments, 200 graduations,1 μm per graduation, 200 μm per rotation, total travel approximately 28 mm. Supplied with an adjustable rack stop to prevent damage to sample and objectives. The coarse adjustments are equipped with friction control
150 x 140 mm stage with integrated X-Y mechanical stage. A ceramic stage is also available on request
Condenser For Bright Light :
Height adjustable Abbe condenser N.A. 1.25 with iris diaphragm. Supplied with a white diffusion filter. Phase contrast rings mounted in slider can be inserted in the condenser for 10x and S40x or 20x and S100x phase contrast objectives. A dark field stop in slider for the 10x, 20x and S40x objectives can also be inserted with magnification indication
Condenser for Fluorescence :
The fluorescence attachment is equipped with an integrated condenser with iris diaphragm and with a field diaphragm
Illumination for Bright Light and phase-Contrast :
All models are supplied with a 3 W adjustable NeoLED illumination with built-in 100-240 V power supply. Köhler illumination with field diaphragm
Illumination for Fluorescence :
Fluorescence attachment with 100 W (HBO) mercury vapor incident epi-illumination and rail for maximum 4 filter blocks. Two general purpose filter sets are standard supplied with the attachment: AE.3245 (Blue excitation) and AE.3246 (Green excitation). Two other general purpose filter sets AE.3246 (UV excitation) and AE.3247 (Ultraviolet excitation) are also available. More specific filter sets are available on request
Filters for Fluoresnce :
Illumination for FLUOLED Attachment :
The FluoLed attachment is standard delivered with a 455 nm LED for efficient and fast detection of tuberculosis with Auramine-O fluorochrome. FluoLED attachments are also available with a 560 nm LED and Green filter set or a 365 nm LED together with an Ultraviolet filter set